Three-Dimensional Structure of Arabidopsis thaliana Lipase Predicted by Homology Modeling Method
نویسندگان
چکیده
Triacylglycerol lipases have been thoroughly characterized in mammals and microorganisms. By contrast, very little is known about plant lipases. In this investigation, a homology model of Arabidopsis thaliana lipase (NP_179126) was constructed using a human gastric lipase (PDB ID: 1HLG), as a template for model building. This model was then assessed for stereochemical quality and side chain environment. Natural substrates: tributyrin, trioctanoin and triolen were docked into the model to investigate ligand-substrate interaction.
منابع مشابه
Comparison of the Lipophosphoglycan 3 Gene of the Lizard and Mammalian Leishmania: A Homology Modeling
Background: Lipophosphoglycan 3 (LPG3) is required for the LPG assembly, a well known virulent molecule. In this study, the LPG3 gene of the lizard and mammalian Leishmania species were cloned and sequenced. A three-dimensional structure (3D) for the target sequence was also predicted by comparative (homology) modeling. Materials and Methods: An optimization PCR amplification was performed o...
متن کاملDifferential Expression of Arabidopsis thaliana Acid Phosphatases in Response to Abiotic Stresses
The objective of this research is to identify Arabidopsis thaliana genes encoding acid phosphatases induced by phosphate starvation. Multiple alignments of eukaryotic acid phosphatase amino acid sequences led to the classification of these proteins into four groups including purple acid phosphatases (PAPs). Specific primers were degenerated and designed based on conserved sequences of PAPs isol...
متن کاملHomology modeling and structure prediction of thioredoxin (TRX) protein in wheat (Triticum aestivum L.)
Wheat is an important dietary cereal often associated with beneficial health effects. A study was carried out to investigate the in silico analysis of homology modeling and 3D structure prediction of Thioredoxin (TRX) protein in Triticum aestivum. Primary structure prediction and physicochemical characterization were performed by computing theoretical isoelectric point (pI), molecular weight, t...
متن کاملYeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
SETH4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of Arabidopsis thaliana. This fragment was PCR amplified using Kod Hi Fi DNA polymerase enzyme. This fragment was cloned into pGBKT7 bate vector and transformed E. coli DH5? cells containing vector were selected on LB medium containing Kanamycin. Finally, pGBKT7-SETH4 bate was transformed into yeast ...
متن کاملمشکلات روشهای موجود و ارائه دو روش جدید کشت هیدروپونیک گیاه آرابیدوپسیس تالیانا
Arabidopsis thaliana is a suitable model plant for genetic and molecular biology studies in higher plants. However, its hydroponic culture for biochemical and physiological studies is a challenge due to small size, capillary roots and little biomass at maturity. Several cultural systems have been suggested for Arabidopsis thaliana hydroponic culture, each having special advantages and disadvant...
متن کامل